Ultrafast laser portfolio for neuroscience and bio-Imaging

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MKS Instruments has announced the expansion of its Spectra-Physics InSight X3+ tunable ultrafast lasers with the introduction of an integrated attenuation option.

This new capability provides on-board, software-controlled adjustment of the output power to augment the built-in automated wavelength and dispersion compensation tuning for added optimisation of multiphoton imaging performance.

Herman Chui, senior director of marketing for the Light and Motion Division of MKS comments: 'With an astounding 950 research publications produced using the InSight family of lasers and over 10,000 citations of these publications, the InSight X3 and X3+ are recognised as the leading lasers for multiphoton imaging used in live deep tissue imaging for Neuroscience and many other applications. The new integrated attenuation option is another significant extension of our ultrafast laser portfolio for bio-imaging applications.’

An essential component for hundreds of bio-imaging facilities worldwide, the InSight laser family offers an impressive 680 to 1300 nm wavelength tuning range, and every InSight laser includes DeepSee automated dispersion compensation to ensure the shortest pulses are delivered to the sample. 

The InSight X3+ model offers more than 3 W at the peak of the tuning range and extends into the 900 to 1100 nm range where many biologically impactful fluorophores have their absorption bands. Entirely software controlled, the InSight X3+ with integrated attenuation is fully equipped and ready to be used in a wide array of applications including multiphoton imaging, Stimulated Raman Spectroscopy (SRS), Second Harmonic Generation (SHG) microscopy, Coherent Anti-Stokes Raman Spectroscopy (CARS), and Fluorescence Lifetime Imaging Microscopy (FLIM).

The InSight family of lasers have passed extensive environmental qualification testing, and each laser is subjected to rigorous production tests to ensure high reliability. The InSight lasers are field serviceable to ensure maximum uptime and to eliminate the need to realign the microscope in the unlikely event of a service call.

Optical benches at Politecnico di Milano, the general coordinator of the Crimson project. Credit: Politecnico di Milano

20 July 2022