The Fluorescence Lifetime (FLIM) upgrade kit from Picoquant has been further improved and is now also available for the new two photon confocal microscope from Olympus, the FluoView FV1000MPE with non-descanned detection (NDD).
The upgrade kit extends the capabilities of the microscope towards time-resolved FLIM measurements with a temporal resolution down to picoseconds and allows users to perform various analysis on this time scale.
The kit includes a small modification to the NDD unit of the Olympus MPE system, which enables the user to easily switch between standard NDD imaging and FLIM via a simple change of cables. Up to 4 internal NDD detectors can be used for FLIM. The kit uses the two photon laser source of the FV1000MPE system as excitation source and is completed by Time-Correlated Single Photon Counting (TCSPC) electronics and a special data acquisition and analysis software for FLIM and FLIM-FRET.
Due to the usage of many components already present, the upgrade kit is an economical way to extend the capabilities of the FV1000MPE towards FLIM measurements. NDD FLIM is extremely suited for deep tissue FLIM, where descanned detection becomesvery insensitive. Still, the FV1000MPE can also be combined with sensitive descanned detection by additionally attaching Single Photon Avalanche Diodes (SPADs) with a extremely high quantum efficiency. This would make additional measurement modes like Fluorescence Correlation Spectroscopy (FCS) possible.