The course is intended for individuals wishing an in-depth introduction to the principles of fluorescence spectroscopy and its applications to the Life Sciences. Attendees are typically professionals who are using or intend to use fluorescence in their research. Most attendees have some knowledge of fluorescence, typically in a specialised area. However, individuals from totally different research areas and from industry get the opportunity to enter this exciting field in a very effective way and will especially benefit from the experimental section.