Light Conversion has introduced Cronus-2P and Cronus-3P microscopy-dedicated femtosecond laser sources covering applications in functional neuroimaging, optogenetics, and deep imaging using medium-repetition-rate three-photon excitation and fast high-repetition-rate two-photon imaging.
Cronus-3P provides μJ-level pulses down to 50fs at repetition rates of up to 2MHz and tunable in the short-wavelength infrared (SWIR) range from 1.25 to 1.8μm, thus covering the biological transparency windows at 1.3μm and 1.7μm for three-photon microscopy.
It achieves tunable femtosecond excitation with an integrated group delay dispersion (GDD) control, ensuring optimal pulse duration at the sample, and industrial-grade design to guarantee excellent short- and long-term output stability.
Cronus-2P is a femtosecond laser providing three simultaneous synchronised outputs with high repetition rate, high output power, short pulse duration, and GDD control, making it ideal for nonlinear microscopy. Two outputs are independently tunable in the 680-960nm and 960-1,300nm ranges, while the third is fixed at 1,025nm.
It can be used for simultaneous excitation of multiple fluorescent probes, calcium indicators, or opsins at their absorption maxima, whereas second- and third-harmonic emission (SHG and THG) can be spectrally shifted for ease of detection or resonant enhancement. The three simultaneous outputs also provide for advanced coherent anti-Stokes and stimulated Raman scattering (CARS and SRS) applications.