Lambert Instruments today has introduced a new fluorescence lifetime imaging microscopy system: The Lambert Instruments FLIM Attachment for Time-Domain imaging. The LIFA-TD offers a turn-key solution for fluorescence lifetime imaging microscopy and is compatible with every widefield fluorescence microscope. From recording the data to calculating the fluorescence lifetime, the entire measurement procedure is automated by advanced software.
The LIFA-TD includes a camera with a built-in image intensifier, which can reduce the exposure time to 3 ns and alter the timing of the exposure in the subnanosecond range. A pulsed laser excites the fluorophores in the sample. By using a laser with a small pulse width, the accuracy of the lifetime measurements is optimized.
Images are recorded with custom software, which also processes the data to determine the fluorescence lifetime. The fluorescence lifetimes are then shown as a colorized overlay on the original image.